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Indian Journal of Veterinary Pathology
Year : 2006, Volume : 30, Issue : 2
First page : ( 10) Last page : ( 13)
Print ISSN : 0250-4758.

Immunohistochemical demonstration of mycobacterial antigens in sheep experimentally infected with Mycobacterium avium subspecies paratuberculosis

Kumar A.A., Tripathi B.N., Jolhe D.K.

Division of Pathology Indian Veterinary Research Institute, Izatnagar-2431002

Corresponding author


The objective of the present study was to compare diagnostic efficacy of indirect immunoperoxidase (IPT) and Ziehl Neelsen's (ZN) methods in histological confirmation of paratuberculosis. Fifteen lambs were orally infected with a caprine isolate of M.a.paratuberculosis. A group of 8 lambs were kept in the contact with the infected group and 8 lambs as uninfected control. The animals of the infected and in-contact groups were classified as suspected (2), mild (9), moderate (3) and severe (3) infection categories on the basis of severity of histological lesions and positivity in other diagnostic tests. Demonstration of acid-fast bacilli and M.a.paratuberculosis antigen in tissues of 15 experimentally infected sheep. Two sheep showing no lesions were negative in both tests. Out of 2 suspected and 9 mild cases, only IPT detected 1 and 6 cases, respectively. In moderate and severe cases, both IPT and ZN methods were equally sensitive with former giving more positive signals than the latter. The overeall sensitivity of IPT (76.5%) was found to be far superior as compared with ZN method (35.3%). The results suggested the utility of IPT in the diagnostic confirmation of histological lesions without demonstrable AFB.


Key words

acid-fast bacilli, diagnosis, immunoperoxidase test, paratuberculosis, Ziehl Neelsen's method.


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