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Year : 2024, Volume : 13, Issue : 1
First page : ( 89) Last page : ( 96)
Print ISSN : 2320-1193. Online ISSN : 2394-448X. Published online : 2024 June 19.
Article DOI : 10.5958/2394-448X.2024.00019.4

Molecular Identification and Characterization of Hollyhock yellow vein virus Associated with Yellow Vein Net & Leaf Curl Disease in Alcea striata L. from Central India

Snehi Sunil Kumar*, Kushvaha Ram Prasad, Parihar Shailendra Singh, Jain Bhoopendra

Molecular Plant Virology Lab, Department of Microbiology, Barkatullah University, Bhopal-462026 (M.P.), India

*Corresponding Author : sunilsnehi@gmail.com

Online Published on 19 July, 2024.

Received:  18  April,  2024; Accepted:  07  June,  2024.

Abstract

A number of plant species are grown in India as ornamentals for their aesthetic values. Several viruses have been reported to cause various diseases on them which affected their quality and production. Naturally infected symptomatic and asymptomatic leaf samples of hollyhock (Alcea striata L.) were collected during a survey in February 2019 for the molecular identification and characterization of begomovirus-associated disease. The hollyhock plant exhibited symptoms of the disease such as yellow vein net, leaf curl, and reduced leaf size on leaves. A population of whiteflies (Bemisia tabaci) was also noticed in the vicinities; therefore, begomovirus infection was suspected. To confirm the presence of virus disease on hollyhock, PCR was performed using three sets of begomovirus genus-specific primers for the complete DNA-A genome and the expected size of the amplicon was successfully amplified. The positive amplicons were purified, sequenced and consensus sequence data of two identical sequences, producing a complete DNA-A genome sequence was deposited in the NCBI GenBank database under accession (MK965197). BLASTn analysis of the virus isolate (MK965197) showed the highest 95% nucleotide sequence identity and close phylogenetic relationships with Hollyhock yellow vein virus (MG373552). On the basis of complete DNA-A genome sequence analysis and close phylogenetic relationships of the under study begomovirus associated with yellow vein net and leaf curl disease of Alcea striata was identified as Hollyhock yellow vein virus first time from, Central India.

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Keywords

Hollyhock, Bemisia tabaci, PCR, Sequence analysis, Phylogenetic relationships, Hollyhock yellow vein virus.

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