Influence of Hormonal and Non-hormonal Therapies on Fertility and Serum Progesterone, Metabolites and Minerals Profile in Anoestrus Crossbred Cows Kumar Rajesh1, Butani M.G.1, Kavani F.S.1, Dhami A.J.*, Shah R.G.2, Sarvaiya N.P.2, Killedar Ankita2 1Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, AAU, Anand 2Reproductive Biology Research Unit, College of Veterinary Science and Animal Husbandry, AAU, Anand *E-mail: dhami_1659@yahoo.com
Online published on 11 January, 2012. Abstract A study was carried out on 31 anoestrus/suboestrus crossbred cows to evaluate the therapeutic efficacy of various hormonal and non-hormonal drugs in inducing fertile oestrus and its influence on serum progesterone, metabolites and minerals profile. Seven true anoestrus cows were treated with GnRH (Receptal 5.0 ml) IM once, eight with Tono-Prepaline-Cyclomin-Lugols (TPCL) 4 times 4–5 days apart, and 10 suboestrus cows were treated with PGF2α (Juramate 2.0 ml) IM once, keeping 6 anoestrus animals as untreated control. The oestrus induction response and the conception rate at induced oestrus in 4 groups were 85.71 and 66.66, 70.00 and 50.00, 90.00 and 55.55, and 33.33 and 50.00%, respectively, with the oestrus induction interval of 31.43±5.61, 24.92±4.72, 3.33±0.53 and 62.57±15.69 days. Overall 74.19% cows responded in 32.09±2.18 days with a conception rate of 54.17%. The serum progesterone profile of TPCL-treated anoestrus cows was significantly higher at the pre-treatment period (1.67±0.31 ng/ml) and at 20–22 days post-AI (4.12±0.76 ng/ml) than at induced oestrus (0.64±0.10 ng/ml). Similarly, it was the trend for GnRH- and PGF2α-treated animals in the control group. However, there was no significant variation in the serum P4 levels between groups at different time intervals, except at pre-treatment period, though the values were higher in suboestrus/PGF2α group. The cows that conceived the following different treatments had significantly higher serum progesterone levels at 20–22 days post-AI as compared to those that did not conceive. The overall mean serum biochemical profile for hormonal and non-hormonal treated anoestrus cows was: Total protein 9.57±0.18 g/dl, total cholesterol 187.89±8.56 mg/dl, calcium 9.98±0.18 mg/dl, phosphorus 8.72±0.15 mg/dl, magnesium 3.64±0.09 mEq/L, zinc 1.04±0.07 ppm, iron 3.06±0.09 ppm, copper 0.64±0.02 ppm, cobalt 0.14±0.01 ppm and manganese 0.08±0.01 ppm, respectively. Neither the pooled values nor the period values of different treatment groups differed significantly. Moreover, the values of protein and cholesterol were slightly higher at oestrus and at 20–22 days post-AI in non-conceived than the conceived animals, but did not vary significantly. The total cholesterol content was relatively higher in suboestrus/PGF2α-treated cows as compared to other treatment groups. Among GnRH- and PGF2α- treated cows, there was a gradually increasing trend for serum calcium and magnesium during the three periods of blood sampling. The zinc showed gradually an increasing trend in TPCL-treated cows, whereas iron had a similar rising trend in the PGF2α-treated group. The serum zinc and copper concentrations were higher, and cobalt and manganese lower in the conceived group as compared to the non-conceived group. Moreover, in the control group, there was a gradually increasing trend in period values for iron and copper. Results proved that though none of the therapies significantly influenced the blood profile of elements studied, they had definite clinical values to manage anoestrus and suboestrus cows under field conditions. Top Keywords Anoestrus cows, Hormonal/Non-hormonal therapy, Fertile oestrus induction, Serum profile, Progesterone, Metabolites, Minerals. Top |