Analysis of inheritance in a novel seedling stage zebra leaf mutant, Pusa Zebra 18 in rice (Oryza sativa L.) Amaresh, Krishnan S. Gopala*, Vinod K. K., Viswanathan C.1, Mithra S.V.A.C.R2, Ellur Ranjith Kumar, Bhowmick P.K., Bollinedi Haritha, Nagarajan M.3, Dhawan Gaurav, Kumar Pankaj, Singh A. K. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India 1Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India 2Division of Molecular Biology and Biotechnology, ICAR-National Institute of Plant Biotechnology, New Delhi, 110 012, India 3IARI-Rice Breeding and Genetics Research Centre, Aduthurai, 621 101, Tamil Nadu, India *Corresponding Author: S. Gopala Krishnan, Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India, E-Mail: gopal_icar@yahoo.co.in
Online Published on 14 August, 2024. Abstract A novel zebra leaf mutant, Pusa Zebra 18 derived spontaneously from F2 intercross population of Pusa 44 near-isogenic lines (NILs) exhibits distinguishable yellowish cross bands across the green leaf blade, specifically expressed in the seedling stage from 6 DAT, which diffuses progressively, leading to the recovery of the normal green leaf by 30 DAT. We elucidated the genetics and nature of inheritance for zebra trait in F2 and BC1 F2 populations by crossing Pusa 6B with Pusa Zebra 18. The leaves of the F1 were a normal green color, indicating that the seedling stage zebra leaf trait in Pusa Zebra 18 is recessive. Chi-square goodness of fit analysis of these segregating populations showed that the segregation ratio was fitting to the Mendelian segregation ratio of 3:1. The seedling stage zebra leaf mutation in Pusa Zebra 18 is monogenic recessive in nature. Further, mapping, fine mapping, cloning, and functional characterization need to be carried out, which will help gain insight into the mechanism involved in chlorophyll biosynthesis and regulation studies in rice. Top Keywords Leaf color, Zebra mutant, Pusa Zebra 18, Inheritance, Segregation. Top |