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Year : 2022, Volume : 10, Issue : 15
First page : ( 176) Last page : ( 182)
Print ISSN : 2349-9885. Online ISSN : 2582-5089. Published online : 2022  16.
Article DOI : 10.5958/2582-5089.2022.00021.4

Effect of different concentrations of BAP and constant NAA on tissue-cultured regeneration of manga Bamboo (Dendrocalamus stocksii)

Tahakik Rushikesh1,*, Maske Nilesh2, Arsode Pandurang3

1Department of Plant Biotechnology, MGM College of Agricultural Biotechnology, MGM Hills Gandheli, Aurangabad-431001, Maharashtra, India

2Department of Agronomy, Nanasaheb Kadam College of Agricultural Biotechnology, MGM Hills, Gandheli, Aurangabad- 431001, Maharashtra, India

3Department of Genetics and Plant Breeding, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India

*Email: rushi.mgmcabt@gmail.com

Online Published on 16 September, 2023.

Abstract

The bamboo plant is an important biodegradable raw material that can play an important role in the rejuvenation of the Indian rural economy by positively impacting agricultural, industrial, energy and environmental sectors. In vitro, bud break and aseptic culture establishment were evaluated for large-scale clonal propagation of the commercially important bamboo species Dendrocalams stocksii Munro. The experiment was conducted twice using a completely randomized design with 6 replicates per treatment. Among various concentrations of BAP and constant NAA, MS media supplemented with BAP 5 mg/L treatment is most congenial for the initiation and establishment of aseptic cultures. 6 Benzyl aminopurine (BAP) without supplementation of any additive resulted in significant bud breakage in the case of D. stocksii. Altered carbon source in MS media resulted in comparatively equal responses, and no significant variation was observed. For multiplication, BAP (5 mg/L) showed a maximum number of shoots (9±2) per explant in D. stocksii. For root initiation, further studies are needed. Well-developed plants were successfully acclimatized, and the success rate was 72%.

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Keywords

Tissue culture, Bamboo, BAP, NAA, Dendrocalamus stocksii Munro.

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