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Year : 2023, Volume : 11, Issue : 1and2
First page : ( 40) Last page : ( 46)
Print ISSN : 2349-9885. Online ISSN : 2582-5089. Published online : 2023  19.
Article DOI : 10.5958/2582-5089.2023.00006.3

Assessment of Azotobacter isolates against sheath blight pathogenic fungus (Rhizoctonia solani) and evaluation of antagonistic activity from infected rice plant

Singh Raj Vardhan*, Shweta

Department of Plant Molecular Biology and Genetics Engineering, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India

*Email: rajvsingh199@gmail.com

Online Published on 19 February, 2024.


The primary objective of the current investigation is that to collect, rice rhizosphere soil sample from different regions of Uttar Pradesh, India. Isolation of 09 Azotobacter isolates of rice rhizosphere was done on Jensen’s medium. The Rhizoctonia solani fungus was isolated from infected plant of rice and identified microscopically. Out of the 09 isolates, only three isolates (1, 2 and 7) showed high inhibitory effect on the pathogenic fungus in dual culture on PDA medium. The screening of 09 rice rhizosphere isolates for Indol acetic acid (IAA) was done in Luria broth (LB) medium. Only one isolates showed highest amount of indole acetic acid production. All isolates were screened for ammonia production and phosphate solubilization and among them only 02 isolates showed highest ammonia production and 04 isolates showed highest amount of phosphate solubilization.



Antagonistic, Assessment, Azotobacter, Fungus, Rice, Sheath blight pathogenic.


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