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Phytopathogenic Mollicutes
Year : 2011, Volume : 1, Issue : 1
First page : ( 35) Last page : ( 39)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/j.2249-4669.1.1.005

Echinacea purpurea- a host of 16SrXII-A phytoplasma group in Serbia

Pavlovic Snezana1, Starovic Mira2, Stojanovic Sasa2, Popovic Tatjana2, Aleksic Goran2, Drazic Slobodan1, Josic Dragana3,*

1Institute for Medicinal Plant Research “Dr. J. Pancic”, Belgrade, Serbia

2Institute for Plant Protection and Environment, T. Drajzera 9, Belgrade, Serbia

3Institute of Soil Science, Genetic laboratory, T. Drajzera 7, Belgrade, Serbia

Corresponding author e-mail: *josicdragana@yahoo.com

Received:  27  March,  2011; Accepted:  12  May,  2011.

Abstract

During a survey carried out from 2005 to 2010, reddening, stunting and proliferation of axillary shoots or witches’ broom symptoms were observed on Echinacea purpurea in Pancevo and Indjija, Serbia. The association of phytoplasma with these symptoms has been detected by electron microscopic observations and PCR followed by nested PCR using phytoplasma 16S rRNA gene specific primers. Further, 16SrXII-A group (stolbur) phytoplasmas were identified by RFLP analysis using TruI, KpnI and AluI restriction endonucleases.

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Keywords

16SrXII-A phytoplasma subgroup, Echinacea purpurea, nested PCR, RFLP.

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