|
|
|
|
|
|
|
|
|
|
Characterization and evaluation of native Trichoderma isolates for antagonistic activity against Fusarium oxysporum f. sp. ciceris Kumari Deepak, Yadav N. K.*, Singh Narender, Saran Mahendra Kumar, Rahul Department of Plant Pathology, CCS Haryana Agricultural University, Hisar, Haryana-125004 *E-mail: yadavnk67@gmail.com
Online published on 10 September, 2024. Abstract Chickpea wilt incited by Fusarium oxysporum f. sp. ciceris is the most destructive seed and soil borne disease which appears every year and causes heavy losses in yield. Present investigation was carried out for in vitro evaluation of Trichodema isolates against F. oxysporum f. sp. ciceris (Foc-49) and to characterize them on morphological basis. Thirteen Trichodema isolates were isolated from rhizosphere samples collected from chickpea growing areas of Haryana. Trichoderma isolates exhibited fastest growth on corn meal agar (5.0-5.9 cm) medium while, slowest growth was recorded on czapek-dox agar (1.1-3.2 cm) medium. Conidial and mycelial size varied with different Trichoderma isolates. Conidial size varied from 7.23 to 18.86 μm whereas, width of mycelia of different isolates of Trichoderma varied from 2.29 to 5.49 μm. Eight isolates showed positive results for HCN production. IAA production levels ranged from 11.48 to 21.78μg/ml for different isolates. The efficacy of these antagonists was investigated by employing the dual plate confrontation assay technique against Foc-49. The mycelial growth inhibition zone varied from 62.2 to 88.1% against F. oxysporum f. sp. ciceris and the highest inhibition (88.1%) was exhibited by HST-1 isolates. Further, the isolates which performed best under in vitro namely HST-1 (ITCC No. 9071) and HMT-2 (ITCC No. 9072) were identified as Trichoderma asperellum on morphological basis. Top Keywords Biocontrol, Chickpea, F. oxysporum f. sp., Ciceris Trichoderma. Top | |
|
|
|
|
║ Site map
║
Privacy Policy ║ Copyright ║ Terms & Conditions ║
 ║
|
|
|
849,211,496 visitor(s) since 30th May, 2005.
|
|
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
|
|
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.
|