In-vitro screening of native strains of fluorescent pseudomonads against Xanthomonas axonopodis pv. punicae, causing bacterial blight of pomegranate Giri Madhu S.1,*, Benagi V. I.2, Nargund V. B.2, Nirmalnath P. Jones3 1Central Coffee Research Institute, Coffee Research Station Post, Chikkamagaluru District-577 117 2Department of Plant Pathology, University of Agricultural Sciences, Dharwad-580 005 3Department of Agricultural Microbiology, University of Agricultural Sciences, Dharwad-580 005 *E-mail: madhuccri@gmail.com
Online published on 10 September, 2024. Abstract Fluorescent pseudomonads isolated from rhizosphere soils of northern Karnataka and border areas of Maharashtra were screened against X. axonopodis pv. punicae, the causal agent of bacterial blight of pomegranate by dual culture technique in-vitro. Out of 99 colonies of fluorescent pseudomonads screened for the antagonistic activity, 83 colonies were found inhibitory against pathogen. The zone of inhibition ranged from 1.47 cm to 4.35 cm. The top nine strains of fluorescent pseudomonads were selected which showed maximum inhibitory zone and where pathogen failed to show re-growth in the inhibitory zone after 144 hours after inoculation. Among the nine selected strains FP-87 showed a maximum zone of inhibition of 4.35 cm in diameter followed by FP-64, FP-19 and FP-03 with a zone of inhibition 4.07 cm, 3.58 cm and 3.48 cm in diameter respectively. However, inhibition zone produced by FP-87 strain was significantly superior over remaining strains except FP-64 where they were at par with each other. Remaining strains were at par with each other. Top Keywords Bacterial blight, Fluorescent pseudomonads, Pomegranate, Xanthomonas axonopodis pv. punicae. Top |