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Research Journal of Pharmacology and Pharmacodynamics
Year : 2024, Volume : 16, Issue : 4
First page : ( 274) Last page : ( 276)
Print ISSN : 0975-4407. Online ISSN : 2321-5836.
Article DOI : 10.52711/2321-5836.2024.00047

Comparative Study of Antibacterial activity of neem leaf extract against standard benzyl penicillin by using In vitro cup plate method

Shaikh Junaid S*, Tadvi Sunil Jabra, Barhate Shashikant D

Shree Sureshdada Jain Institute of Pharmaceutical Education and Research, Jamner, Jalgaon, Maharashtra, India

*Corresponding Author E-mail: skjunaid.pharm@gmail.com

Online Published on 23 April, 2025.

Abstract

This research article is based on the antibacterial activity of neem. We compared the antibacterial activity of a standard antibiotic with our neem alcoholic extract using the cup-plate method. For this study, we utilized non-pathogenic bacteria, specifically Lactobacillus grown in MRS agar media prepared with tomato juice and meat extract. This bacterial culture was employed to evaluate the antibacterial activity of neem leaf extract. The entire study was conducted in a college laboratory to understand the concepts of microbial assay and the format of research paper writing. The benefit of using neem extract lies in its easy availability and low cost. However, neem is not used commercially due to its bitter taste and the challenging extraction process.

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Keywords

Neem leaf extract, Benzyl Penicillin, Cup plate method, Lactobacillus.

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