The Estimation of Irinotecan HCl in Parenterals by RP-HPLC. Kumar V Kiran1, Raju N Appala2,*, Rani Namratha2, Rao JVLN Seshagiri3, Satyanarayana T2 1Dept. of Pharma. Chem., Sree Chaitanya Institute of Pharmaceutical Sciences, LMD.Colony, Karimnagar-505527, AP 2Dept. of Pharma. Chem., Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road# 3, Banjara Hills, Hyderabad-500 034 3University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam. AP *Corresponding Author E-mail: kiranphd@yahoo.com
Online published on 13 March, 2013. Abstract A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Irinotecan HCl in tablet dosage form. A X-Terra RP C18, 250x4.6 mm i.d, 5 μm partical size, with mobile phase consisting of Methanol and 0.01 M Ammonium Acetate containing 0.1% formic acid and methanol in the ratio of 50:50 v/v was used. The flow rate was 1 ml/min and the effluents were monitored at 250 nm. The retention time was 4.69 min. The detector response was linear in the concentration of 120–360 mcg/ml. The respective linear regression equation being Y=166582.24x+86439.5. The limit of detection and limit of quantification was 0.06 and 0.18 mcg/ml respectively. The percentage assay of Irinotecan HCl was 99.09%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Irinotecan HCl in bulk drug and in its pharmaceutical dosage form. Top Keywords Irinotecan HCl, RP-HPLC, Estimation, and Tablets. Top |