Genotypic characterization of Pasturella multocida isolated from an outbreak of fowl cholera Kurkure N.V.3,*, Hedau Madhuri3, Raut S.S.1,3, Kalorey D.R.2,3, Bhandarkar A.G.3 3Department of Pathology, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University (MAFSU)Nagpur-440 006 2Department of Microbiology, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University (MAFSU)Nagpur-440 006 1division of Pathology, Indian Veterinary Research Institute, Izatnagar-243122 (U.P) *Corresponding author: e-mail: nitinkurkure@rediffmail.com
Abstract The mortality was reported in poultry flock at about 0.5% daily for 12 days. The necropsy findings revealed mild cyanotic comb and wattles, petechial haemorrahges on the serous membranes, congestion and oedema of lungs. Haemorrahges were also evident on the ovaries. Histologically, lungs revealed congestion, oedema and interstitial pneumonia. Congestion of central vein, haemorrhages in hepatic lobules and hepatocytes revealed granular degeneration and necrosis. Kidneys revealed haemorrhages and necrosis of tubular epithelium. Brain sections revealed congestion and oedema of parenchyma. The bacterial isolates were found to be Gram-negative coccobacilli. The PCR amplified products of ∼ 460 bp size (PM-PCR), and ∼ 1044 bp (CAPA) in multiplex PCR determined the specificity of P. multocida serogroup A. The PM-PCR assay used in this study was highly specific and sensitive providing rapid detection of P. multocida regardless of the purity of the sample. Also the direct PCR employed on clinical samples will help in early diagnosis of the disease. Top Keywords Fowl cholera, Genotype, Outbreak, Pasteurella multocida. Top |