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Year : 2022, Volume : 10, Issue : 15
First page : ( 54) Last page : ( 60)
Print ISSN : 2349-9885. Online ISSN : 2582-5089. Published online : 2022  16.
Article DOI : 10.5958/2582-5089.2022.00007.X

Molecular diagnosis of ‘Candidatus Phytoplasma australasia’ related strain (16SrII-D) associated with flat shoot and shoot proliferation of radish in Uttar Pradesh, India

Singh Noopur1, Dubey Durgesh Kumar2, Singh Sushil Kumar3, Rao Govind P.4,*

1Centre of Genomics and Bioinformatics, Institute of Agriculture and Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India

2Department of Plant Pathology, Institute of Agriculture & Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India

3Department of Plant Pathology, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India

4Institute of Agriculture and Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India

*Email: gpraossrp@gmail.com

Online Published on 16 September, 2023.

Abstract

Radish is an important vegetable crop used in our daily dishes as salad, vegetable and other common cuisines. In the present study, phytoplasma associated with the flat shoot and shoot proliferation son radish plants grown at Narendra Dev University of Agriculture & Technology, Ayodhya campus, India were attempted. The DNA was extracted from diseased and healthy shoots and subjected to nested PCR assays with P1/P7 and R16F2n/R16R2 phytoplasma specific primer pairs. Amplification of 1250 bp product was achieved in all the symptomatic radish samples. The amplified product was cloned and directly sequenced. Sequence analysis of 16Sr RNA gene through BLASTn, phylogeny and virtual RFLP comparison confirmed radish isolate as Ca. P. Australasia related strain (16SrII-D sub group). To our best knowledge, this is the first report of the 16Sr II-D subgroup phytoplasma associated with radish in India.

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Keywords

Candidatus, Diagnosis, Molecular, Phytoplasma, Proliferation, Radish.

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