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Phytopathogenic Mollicutes
Year : 2023, Volume : 13, Issue : 2
First page : ( 213) Last page : ( 217)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2023.00084.1

First report of phytoplasma presence in a phyllody disease of buckwheat

Bandakkanavar Mahalingappa1, Kedarnath2,*, Hurakadli Manjunath3, Achari Raghavendra4, Ramappa Honnaghatta Krishnappa1, Jagadeesh Thejaswi Kumar5

1Department of Plant Pathology, UAS, GKVK, Bengaluru-560065, India

2ICAR-Krishi Vigyan Kendra(D.K) Mangaluru-575002, KVAFSU, Bidar, India

3CCS Haryana Agricultural University, Hisar-125004, Haryana, India

4College of Horticulture, Munirabad-583233, UHS, Bagalkot, Karnataka, India

5Department of Agricultural Economics, UAS, GKVK, Bengaluru-560065, India

*Corresponding author e-mail: Kedarnath Govin (kedarnath.govin@gmail.com)

Online Published on 11 January, 2024.

Abstract

Buckwheat is an ancient herbaceous pseudocereal crop belonging to Polygonaceae family which occupy a crucial part of the human diet and is consumed globally. Typical symptoms of a phytoplasma disease were collected from buckwheat grown during October 2019 with a disease incidence of 10 to 15% in an experimental field at University of Agricultural Sciences, GKVK, Bengaluru, India. Symptomatic and asymptomatic plant samples were collected, and the DNA was extracted using a CTAB method. A nested-PCR assay was used to amplify the 16S rRNA region of phytoplasmas with the primer pairs P1/P7 and R16F2n/R16R2. All the symptomatic plants and the positive control produced an expected amplicon size of about 1.2-kb, but no products were obtained from the asymptomatic plants. PCR products were cloned and sequenced from both ends. BLASTn analysis of 16S rDNA sequence showed 99.49% nucleotide identity with ‘Candidatus Phytoplasma australasiae = australasiaticum’. This is the first report of phytoplasma presence in buckwheat.

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Keywords

Nested PCR, Phylogenetic analysis, 16S rRNA gene, Molecular identification.

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