(3.238.116.201)
[ij] [ij] [ij] 
Email id
 

Phytopathogenic Mollicutes
Year : 2023, Volume : 13, Issue : 2
First page : ( 224) Last page : ( 227)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2023.00086.5

Molecular characterisation of a ‘Candidatus Phytoplasma australasiae’ strain associated with Jasminum sambac phyllody in India

Reddy Madem Gurivi1,*, Arunodhayam Kalleti2, Sandhya Gopisetty3, Mamatha Neelaturu Chandrashekar1, Jyosthna Mudigulam Karnam1

1Department of Plant Pathology, Sri Venkateswara Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati-517502, Andhra Pradesh, India

2College of Horticulture, Dr. Y.S.R.H.U., Anantharajupeta-516105, Andhra Pradesh, India

3Department of Horticulture, Sri Venkateswara Agricultural College, Acharya N.G. Ranga Agricultural University, Tirupati-517502, Andhra Pradesh, India

*Corresponding author e-mail: Madem Gurivi Reddy (m.gurivireddy@angrau.ac.in)

Online Published on 11 January, 2024.

Abstract

Symptoms of yellowing and little leaf were observed in jasmine plants at farmer fields of Kancharapalem village, Varadaiahpalem Mandal, Tirupati, India. DNA was extracted from symptomatic and asymptomatic leaves and subjected to nested PCR assay with P1/P7 and R16F2n/R16R2 primers and secAfor1/sArev3 and SecAfor2/SecArev3 primers. The PCR amplified 1,250 bp and 480 bp products were eluted, sequenced and the aligned sequences obtained were submitted to NCBI GenBank. Sequence analysis of 16Sr RNA and SecA genes through phylogeny and virtual RFLP of jasmine phyllody phytoplasma allows its identification as ‘Candidatus Phytoplasma australasiae’ and its assignment to 16SrII-D subgroup, respectively.

Top

Keywords

Jasmine, Phytoplasma, 16SrII-D subgroup, Disease.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
772,723,953 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.