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Phytopathogenic Mollicutes
Year : 2019, Volume : 9, Issue : 1
First page : ( 201) Last page : ( 202)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2019.00101.4

Preliminary evaluation of the use of an antiserum obtained from phytoplasma culture

Contaldo Nicoletta1,*, Freo Isabella1, Elicio Vito2, Lucchese Carla1, Formica Lilia2, Bertaccini Assunta1

1Alma Mater Studiorum, University of Bologna, Department of Agricultural and Food Sciences, Bologna, Italy

2Agritest srl, Valenzano, Bari, Italy

*Corresponding author e-mail: Nicoletta Contaldo (nicoletta.contaldo2@unibo.it)

Online published on 25 July, 2019.


The development of methods for culturing phytoplasmas in artificial media suggested the evaluation of strategies to produce antibodies against a phytoplasma target. To this purpose, an aster yellows phytoplasma isolate obtained from field collected infected grapevine was used for the production of a polyclonal antiserum. It was then tested by ELISA and IFAS methods in order to check and evaluate its suitability for phytoplasma detection in plant samples.



Aster yellows isolate, polyclonal antiserum, DAS ELISA, IFAS.


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