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Phytopathogenic Mollicutes
Year : 2019, Volume : 9, Issue : 1
First page : ( 201) Last page : ( 202)
Print ISSN : 2249-4669. Online ISSN : 2249-4677.
Article DOI : 10.5958/2249-4677.2019.00101.4

Preliminary evaluation of the use of an antiserum obtained from phytoplasma culture

Contaldo Nicoletta1,*, Freo Isabella1, Elicio Vito2, Lucchese Carla1, Formica Lilia2, Bertaccini Assunta1

1Alma Mater Studiorum, University of Bologna, Department of Agricultural and Food Sciences, Bologna, Italy

2Agritest srl, Valenzano, Bari, Italy

*Corresponding author e-mail: Nicoletta Contaldo (nicoletta.contaldo2@unibo.it)

Online published on 25 July, 2019.

Abstract

The development of methods for culturing phytoplasmas in artificial media suggested the evaluation of strategies to produce antibodies against a phytoplasma target. To this purpose, an aster yellows phytoplasma isolate obtained from field collected infected grapevine was used for the production of a polyclonal antiserum. It was then tested by ELISA and IFAS methods in order to check and evaluate its suitability for phytoplasma detection in plant samples.

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Keywords

Aster yellows isolate, polyclonal antiserum, DAS ELISA, IFAS.

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