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BIOINFOLET - A Quarterly Journal of Life Sciences
Year : 2024, Volume : 21, Issue : 1
First page : ( 145) Last page : ( 148)
Print ISSN : 0973-1431. Online ISSN : 0976-4755.
Article DOI : 10.5958/0976-4755.2024.00045.2

Molecular identification of bacterial isolates through 16S RRNA gene sequencing and phylogenetic analysis

Chandy Sneha Achamma, Swamy G. E. Mallikarjuna1, Sen Punam

Department of Microbiology, PSG College of Arts & Science, Coimbatore-641014, Tamilnadu

1Department of Forest Pathology, Kerala Forest Research Institute, Peechi-680653, Kerala

Online published on 26 March, 2024.

Abstract

Two bacterial isolates were obtained from rhizosphere soil of Chloris barbata. Genomic DNAwas extracted from each bacterial isolate and 16S rRNA was amplified through PCR using specific primers. Sequencing of the PCR products was carried out. Phylogenetic tree was constructed using the neighbor joining method. The sequence of isolate 1 showed 98.33% similarity with Staphylococcus hominis strain 309-4 while that of isolate 2 showed 98.42% similarity with Bacillus proteolyticus strain H8.

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Keywords

Rhizosphere, Microbiome, Rrnagene, Primers, PCR.

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