Animal Nutrition and Feed Technology

SCOPUS
  • Year: 2023
  • Volume: 23
  • Issue: 2

Binding efficacy and prebiotic properties of commercial yeast cell walls toward aflatoxins and pathogenic E. coli and Salmonella spp.

  • Author:
  • Zeinab Takalloo1, Robabeh Nemati1, Mohsen Nikkhah1, Reza H. Sajedi1,2,*
  • Total Page Count: 16
  • Page Number: 205 to 220

1Kimiazyme Company, Modares Science & Technology Park, Tarbiat Modares University, Tehran, Iran

2Department of Biochemistry Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Department of Biochemistry Faculty of Biological Sciences, Tarbiatmodares University, Tehran-14115-154, Iran

*Corresponding author: sajedi_r@modares.ac.ir

Online published on 31 May, 2023.

Abstract

Yeast cell wall (YCW) fractions are able to bind mycotoxins and pathogenic bacteria and therefore limit their bioavailability in the digestive tract of animals. Most of the available commercial YCW products in the livestock market are processed to achieve specific properties to use as toxin binders or prebiotics. Generally, due to the product processing and differences in product properties, it is difficult to find an efficient singleYCW product in adsorbing mycotoxins and pathogens as well. This study aimed to compare the in vitro capacity of three commercially available yeast cell wall products used as dietary supplements. Accordingly, the adsorption test was performed using aflatoxins and results showed that all YCWs were able to capture the toxins, but to different degrees. In addition, qualitative agglutination test was performed to check the binding capacity of the YCW products for pathogenic E. coli and Salmonella strains. Results showed that all added YCWs were able to form clumps with the strains, but YCW1 (KimiaMOS®) showed a higher adherence to the pathogenic bacteria, andYCW2 (SafMannan®) and YCW3 (TechnoMOS®) also showed some agglutinations to different degrees. Moreover, results from colony forming unit (CFU) assay showed that YCW1 was more efficient in adhering pathogenic bacteria, however, it showed a lower optical density for both bacteria strains during the microplate binding assay. Besides, YCW3 showed a greater adherence capacity for pathogenic bacteria. The results from this study can provide a guideline for quality control of yeast cell wall products used in feed industry.

Keywords

Aflatoxins, Animal feed, Pathogenic bacteria, Prebiotics, Yeast cell walls