Fructosylated human placental DNA recognize circulating autoantibodies from diabetic patients

The main aim is to study the susceptibility of human DNA to non-enzymatic attack by fructose and to ascertain the possible role of fructated DNA in diabetes mellitus patients. To characterize the modified DNA, spectral, electrophoretic, circular dichroism, thermal denaturation was employed. Serum samples from 20 diabetes patients were collected and the binding characteristics of circulating autoantibodies in sera of diabetic patients against native, fructated and ROS-modified fructated DNA were assessed. Immunological techniques employed for the same were direct binding ELISA, competitive ELISA and gel retardation assay. Electrophoresis revealed a decrease in mobility of fructated DNA as compared to native DNA. Results employing physio-chemical techniques demonstrate that human DNA is modified with fructose and ROS, the most damaging species. Binding of DM sera autoantibodies towards fructated and ROS-modified fructated DNA was observed.