Seed storage protein profiling of sulphur applicated chickpea varieties grown under rainfed condition

Chickpea (Cicer arientinum L.) is an important legume crop in India. An experiment was conducted to determine the seed storage protein profiles of five chickpea varieties JG-11, JG-315, PUSA-1103, DCP 92-3 and KGD-1168 at three different levels of sulphur such as S₀, S₁₅ and S₃₀ kg/ha in the form of gypsum grown under rainfed conditions. Analysis was based on biochemical marker sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The results of SDS-PAGE for seed storage proteins showed 19 polypeptide bands with molecular weights of the resolved peptides from 97.4 kDa to 14.3 kDa. Out of these, ten polypeptides having molecular weights 97.4, 66, 46, 19, 18, 16.8, 16.4, 6.2, 6 and 5.5 kDap were universally present, while rest 09 polypeptide bands varied in their expression. The number of bands observed ranged from 14 to 18. The maximum number of polypeptide band was recorded in variety JG-11 by the application of 15 and 30 kg S/ha, while minimum number of polypeptide band was recorded in variety KGD-1168 without application of sulphur. Based on analysis of cluster, it was clear that variety JG-315 without application of sulphur (control) and KGD-1168 with the application of 15 kg S/ha had most distinct bands compared to other varieties. It was concluded that JG-315 and JG-11 varieties by the application of 15 and 30 kg S/ha were found to be suitable for identifying seed storage protein profiles which could be useful in the studies of genetic diversity.