Division of Plant Biotechnology, ICAR-Indian Institute of Pulses Research, Kanpur, 208 024
*E-mail: adas@icar.org.in
Online published on 23 March, 2018.
This is a comparative study of in vitro regeneration potential of two popular Indian desi chickpea cultivars (JG 11 and DCP92-3). Organogenic ability based on Axillary Meristem Explants (AMEs) of both the cultivars were assessed based on pre-standardized regeneration system of chickpea with employing shoot induction and elongation medium (SIM &SEM). The desi cultivar DCP 92-3, responded better in terms of number of potent AMEs prepared (77.75±4.78) fromgerminated seedlings, regeneration response from AMEs (83.53±3.00) and number of shoots per explant (11.08±0.19)as compared to JG 11 (4.48±0.44). Further, separated individual shoots of DCP 92-3 exhibited more elongation (1.54±0.23 cm) as compared to their counterparts derived from JG 11 (0.675±0.09 cm), facilitating micro-grafting andsubsequently higher plant establishment. The study indicates the amenability of specific chickpea cultivar for in vitro regeneration that could be used for genetic transformation studies.
