In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp] using various explants

An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties (BDN-2 and GT-101). Three different explants viz., leaf, hypocotyl and root excised from 15–20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. Shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine. Elongated shoots were transferred in MS + 1.0 mg/l IAA media for rooting. Rooted plants were transferred in pot for acclimatization.