Oxidative stress and antioxidative enzymes activity in pigeonpea leaves at different stages of development under waterlogging, salinity and combined stress of waterlogging and salinity

Four pigeonpea genotypes (ICPH 2431, PARAS, UPAS 120 and H09 33) were raised in polythene bags filled with half kg soil + FYM manure mixture. Water logging, salinity and waterlogging + salinity treatments were given for 8 days and observations were recorded at initial stage 1^st day and subsequently after 8 days of treatment (20 and 40 day old plant leaves). A significant increase in superoxide radical content was observed with all the three treatments both 1 and 8 days after removal from treatment. However increase was comparatively less 8 day after removal from treatment. Among all the treatment salinity alone was found least deleterious and combined waterlogging and salinity was found more deleterious. Increase in antioxidative enzyme activity and antioxidative metabolite content was also observed in all the treatments. Maximum increase was observed in ICPH 2431 followed by PARAS, HO 933and minimum was observed in UPAS 120.