1Shaheed Udham Singh College of Research and Technology, Tangori-140 306, Mohali, Punjab, India
2Department of Zoology, Sant Baba Bhag Singh University, Jalandhar-144 030, Punjab, India
*Corresponding author: Email: adidhanraj464@gmail.com
Online published on 28 January, 2019.
The present study was planned to examine the pectinase production in bacterial species. Citrus wastes and soil samples were processed for the isolation of bacterial species using Hankin's medium. Morphological and biochemical characterization was carried out from purified cultures that showed clear zones in the Hankin's medium plate. The highest pectinase producing Bacillus sp.2 was selected for comparison of enzyme activity between the free and immobilized cell states. Increased enzyme activity was observed in free cell state as compared to the immobilized cell condition after 48 h and 72 h of incubation. However, pectinase production was higher in immobilized cell condition as compared to the free cell state after 96 h of incubation. Agroindustrial waste such as peel of citrus fruits, rotten vegetable wastes, molasses, etc. could be explored for isolating pectinase producing bacteria
Bacillus sp., pectinase, characterization, citrus fruit
