Advances in Applied Research
  • Year: 2024
  • Volume: 16
  • Issue: 2

Agroinoculation technique for identification of MYMV resistant donors in Black gram and Green gram

  • Author:
  • P. Ahila Devi1,*, L. Gnanasing Jesumaharaja2, E. Rajeswari3, A. Anuradha1, G. Ravi1
  • Total Page Count: 15
  • Page Number: 50 to 64

1Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Nagapattinam - 611 002, Tamil Nadu, India

2Department of Plant Pathology, Uttar Banga Krishi Viswavidhyalaya, Coochbehar - 736 165, West Bengal, India

3Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India

*Corresponding author Email : ahila.devi1@gmail.com

Online Published on 15 October, 2024.

Abstract

Black gram (45 Nos.) and green gram (51 Nos.) entries were subjected to agroinoculation to confirm their level of resistance/susceptibility against Mung bean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that nine entries of green gram (MLT-GG-S-19-07, MLT-GG-S-19-08, MLT-GG-K-19-22, MLT-GG-K-19-26, MLT-GG-R-19-03, MLT-GG-R-19-04, MLT-GG-R-19-05, MLT-GG-R-19-21 and MLT-GG-R-19-28) and thirty five black gram entries (MLT-BG-S-19-01, MLT-BG-S-19-02, MLT-BG-S-19-03, MLT-BG-S-19-04, MLT-BG-S-19-06, MLT-BG-S-19-09, MLT-BG-S-19-10, MLT-BG-K-19-01, MLT-BG-K-19-02, MLT-BG-K-19-03, MLT-BG-K-19-04, MLT-BG-K-19-06, MLT-BG-K-19-09, MLT-BG-K-19-10, MLT-BG-K-19-11, MLT-BG-K-19-12, MLT-BG-K-19-13, MLT-BG-K-19-21, MLT-BG-K-19-24, MLT-BG-K-19-25, MLT-BG-K-19-24, MLT-BG-K-19-25, MLT-BG-K-19-28, MLT-BG-R-19-01, MLT-BG-R-19-02, MLT-BG-R-19-03, MLT-BG-R-19-04, MLT-BG-R-19-05, MLT-BG-R-19-06, MLT-BG-R-19-07, MLT-BG-R-19-08, MLT-BG-R-19-09, MLT-BG-R-19-10, MLT-BG-R-19-12 and MLT-BG-R-19-13) were found to be resistant to YMV. The black gram (5 Nos.) and green gram (17 Nos.) entries that showed resistance under field conditions was found to be susceptible to Yellow mosaic virus disease when subjected to agroinoculation technique. The susceptibility indicated the presence of latent infection in the entries, which failed to express the symptoms under field conditions. The induction of defense mechanisms in the MLT (multilocation trial) entries during Kharif, Rabi and Summer, 2019 revealed that one entry (MLT-BG-R-19-25) produced higher levels of phenol (2.30 g-1 of fresh tissue), polyphenol (0.181 ∆A min-1 g-1), peroxidase (0.133 ∆A min-1 mg-1) and phenyl alanine ammonia lyase (52.5 n mol trans cinnamic acid min-1 g-1). The screening results concluded that the resistant entries can be utilized as a donor in disease resistance breeding programme for generating MYMV resistant progenies in future.

Keywords

Black gram, Green gram, Agroinoculation, Host plant resistance