Advances in Applied Research
  • Year: 2013
  • Volume: 5
  • Issue: 1

Separation of Steroidal saponins from Chlorophytum borivilianum by high performance liquid chromatography

  • Author:
  • Arunabh Joshi, G. Rajamani, Vineet Kaswan
  • Total Page Count: 3
  • Page Number: 78 to 80

Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur - 313 001, Rajasthan, India

*Corresponding author: Email: arunabhjoshi@gmail.com

Online published on 7 April, 2014.

Abstract

Quantification of the furostanol and spirostanol steroidal saponins in the root of Chlorophytum borivilianum was carried out by High Performance Liquid Chromatography (HPLC). The results showed that this method could be used to identify the steroidal saponins in Chlorophytum borivilianum, which is sensitive and quick. Under optimized conditions, the active compounds, borivilianosides extracted from Chlorophytum borivilianum roots were found to be resolved into four furostanol (A to D) and four spirostanol (E to H) steroidal saponins respectively in the HPLC-UV detector system

Keywords

Borivilianosides, Chlorophytum borivilianum, furostanol saponin, HPLC, spirostanol saponin