Advances in Applied Research
  • Year: 2013
  • Volume: 5
  • Issue: 2

Effect of laccases from white rot fungi on the nutritional composition and in vitro digestibility of ragi straw (Eleusine coracana)

  • Author:
  • Vidya Pradeep Kumar1, Chandrashekar Naik2, Manpal Sridhar1,
  • Total Page Count: 7
  • Page Number: 124 to 130

1Bioenergetics and Environmental Sciences Division National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore - 560 030, Karnataka, India

2Sir. M. Visvesvarya Institute of Technology, Hunsamaranhalli, Bangalore – 562 157, India

*Corresponding author: Email: manpalsridhar@yahoo.co.uk

Online published on 7 April, 2014.

Abstract

The effect of laccase enzyme produced by three wild isolates of white rot fungi NI-09, Nl-07 and NI-04 was evaluated for in vitro digestibility of Eleusine coracana (ragi straw). The mycelia of the three selected cultures were immobilized on polyurethane cubes (PUF), which showed increased specific activity of 17298 for NI-07, 13590 for NI-09 and 13627 for NI-04 on the 15th day of culture. The ragi straw after treatment with these laccases showed reduction in the Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF) and the Acid Detergent Lignin (ADL) contents in comparison with untreated straw. The laccase has also induced in vitro digestibility (IVDMD) of the ragi straw. Results from these studies suggest that, laccase sources from the immobilized fungi on PUF cubes can be explored as a digestant material for the straw, an animal feed

Keywords

Laccase, immobilization, digestibility, ragi straw, white rot fungi