1Department of Agricultural Biotechnology and Molecular Biology (FBS & H), Rajendra Agricultural University, Pusa, Bihar – 848125, India
2Department of Plant Pathology, College of Agriculture (Jawahar Lal Nehru Krishi Vishwa Vidyalaya), Jabalpur, Madhya Pradesh – 482 004, India
**Corresponding author: Email: sanjeevcoa@gmail.com
Online published on 7 April, 2014.
A protocol was developed for in vitro propagation of Rauwolfia serpentina L., using leaf explants cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of IAA, BAP and KIN. The callus mediated caulogenesis (72.7%) was observed on medium containing IAA (2.0 mg¯1) and BAP (3.0mg−1). The direct caulogenesis (70.4%) was found on the medium BAP (1.5 mg−1) and KIN (1.5 mg−1). The regenerated shoots were transferred to rooting medium containing IBA (0.5 mg−1). After root proliferation, the in vitro derived plantlets were acclimatized and transplanted successfully into the field condition.
