Analysis of sperm and relationship between conventional sperm parameters and hypo-osmotic swelling test/acrylamide penetration assay -crossbred cattle bulls

This study has investigated the relationship between freshly ejaculated sperm (crossbred cattle bull semen) membrane integrity and viability/percent progressive motility and also between acrylamide penetration assay (APA) and membrane integrity/viability/percent progressive motility. Positive correlation was observed with all the tested parameters. APA was objectively analyzed on the basis of both the sperm number that migrates in upper 0.5 cm and the distance penetrated by the spermatozoa up to the peak point. The correlations were positive for HOST and percent progressive motility/viability. However, the parameters HOST and viability correlated well (r = 0.96) when compared to HOST and motility (r = 0.72). In addition, the percent progressive motility, viability (%), HOST (%) correlated with the distance penetrated by spermatozoa in the acrylamide gel (cm/30 min) and the number of sperms penetrated in upper 0.5 cm/30 min. The correlation was positive and significant. Correlation analysis has revealed that the correlation between acrylamide penetration assay and HOST and viability was higher (r = 0.90/0.91, r = 0.94/0.88) than percent progressive motility (r = 0.60/0.73). The assessment of the quality of the semen based on these parameters has indicated that 57.1% of the samples tested were fit for the desired operations