1Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamilnadu, India
2Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram-608 002, Tamilnadu, India
*Corresponding author: Email: spsarathyagri@gmail.com
Online published on 25 January, 2016.
The present investigation was carried out to elucidate the mechanism of disease resistance of blackgram to powdery mildew by exogenous supply of plant activators. Induction of major defence enzymes such as peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were studied in black gram plants sprayed with plant activators. Increased activity of PO, PPO and PAL was observed in blackgram plants treated with a combined application of acetylsalicylic acid (50 mg L"1) and salicylic acid (50 mg L"1) when compared to untreated control. The enzyme activities increased on the 3"1 day, reached maximum levels from the 5th to 7th day and declined gradually thereafter. Similarly, native polyacrylamide gel electrophoresis (PAGE) revealed that one to five isoforms of the defence related enzymes with a higher intensity were expressed in these treatments
Blackgram, enzymes, plant activator, powdery mildew, salicylic acid
