1Department of Environmental Sciences, Bharathiar University, Coimbatore-641 046, Tamilnadu, India
2Division of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Mumbai-400 061, Maharashtra, India
3PSGR Krishnammal College for Women, Coimbatore-641 004, Tamilnadu, India
4Department of Zoology, Maharshi Dayanand College, Mumbai-400 016, Maharashtra, India
*Corresponding author: Email: lprkrama@gmail.com
Online published on 2 August, 2016.
The present laboratory study was conducted to assess the combined effect of increasing temperatures (26, 31,33 and 36°C) and a sub lethal chlorine concentration (0.1 mg L"1) on the acetylcholine esterase (AChE) activity and histology of brain and muscle in Labeo rohita. Fish were exposed to increasing temperatures only (control group) and in combination to chlorine (test group) for 30 days. The AChE activity significantly decreased in the brain and muscle with increasing temperatures in the test group as compared to the control group. Histopathological changes such as vacuolization, gliosis and aggregation of glial cells were observed in the brain tissue with increasing temperatures in the test group and at elevated temerpature in the control group. No histological changes were noticed in the muscle tissues. Overall results indicate that increasing temperatures augment the effects of sub-lethal chlorine in Z,. rohita.
Temperature, chlorine, Labeo rohita, AChE, histopathology
