Applied Biological Research

In vitro regenerative competence of foliar explants of Coelogyne suaveolens was tested on MS medium containing 3% sucrose, 0.1% activated charcoal (AC), and 6 μM NAA + 9 μMBA wherein ~73% cultures responded positively. About 75% cultured leaf segments of T. latifolia responded positively and formed as many as 5 shoot buds within 5 weeks on MS medium containing 3% sucrose, 0.1% AC, and 3 μM IAA + 6 μMBA. The shoot buds developed from leaf segments of C. suaveolens differentiated into rooted plantlets on ½MS medium containing 3% sucrose, 0.1% AC, and 6 μM NAA + 9 μMBA. In T. latifolia a similar response was registered on MS medium containing 2% sucrose, 0.1% AC and 3 μM NAA + 9 μMBA. The regenerated plants were hardened suitably before transferring to community potting mix where they registered 75–80% survival in the potting mix after two months of transfer.