Applied Biological Research

  • Year: 2014
  • Volume: 16
  • Issue: 2

Bioprocessing of bajra straw using locally isolated Aspergillus niger HD-6 for endocellulase production

Biotechnology Department, Anand Mercantile College of Science, Management and Computer Technology, Near New S.T. Station, Bhalej Road, Anand, Gujarat – 388 001 (India)

1Department of Microbiology and Biofertilizer Project, B.A. College of Agriculture, Anand Agriculture University, Anand, Gujarat-388 001 (India)

Abstract

Aspergillus niger strain HD-6, isolated from Anand, Gujarat (India), was evaluated for endocellulase enzyme production through solid state fermentation (SSF) using bajra straw as main carbon source. Various cultural conditions such as temperature, pH, moisture content, incubation period, nitrogen source, inoculum age and size, etc. were standardized for optimum enzyme production. Using 3 g bajra straw as sole carbon source maximum endocellulase production from 96 hr old A. niger HD-6 was achieved at pH 5.0, incubation temperature 28°C and moisture content 65% after incubation for 4 days.

Keywords

Bajra straw, endocellulase, optimization, solid state fermentation