Applied Biological Research
  • Year: 2017
  • Volume: 19
  • Issue: 2

Isolation and characterization of native fluorescent Pseudomonas from rice fields of Assam (India)

  • Author:
  • Pranamika Sharma, Lohit Ch. Bora, Kamalakshi Devi, Sumita Acharjee, Polash Deb Nath
  • Total Page Count: 10
  • Page Number: 146 to 155

Department of Plant Pathology, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785 013, Assam (India)

*e-mail: sarmahpranamika@gmail.com

Online published on 14 October, 2017.

Abstract

In present study ten isolate of Pseudomonas, isolated from rice rhizosphere, were characterized on the basis of morpho-biochemical and molecular characteristics. Screening of their plant growth promoting activities viz., siderophore production, proteolytic activity, ammonia production, IAA production and HCN production revealed that the isolate PfAs1 was most promising one with highest siderophore production (6.18 cm dia. of yellow halo) and proteolytic activity (5.85 cm dia. halo zone). The results showed that none of the isolates possessed all the tested PGP activities. IAA and ammonia production was determined quantitatively for all the isolates. The IAA and ammonia production in tested Pf strains ranged from 15.66 to 48.58 and 179.05 to 394.72 mg L−1. The phylogenetic analysis using 16S rRNA gene

Keywords

PGPR, proteolytic activity, Pseudomonas, rhizosphere, rice, siderophore