1Department of Biotechnology, College of Horticulture, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-173 230, Himachal Pradesh (India)
2Department of Tree Improvement and Genetic Resources, College of Forestry, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-173 230, Himachal Pradesh (India)
*E mail: 1992samritisharma@gmail.com
Online published on 23 October, 2018.
Molecular markers have proved a valuable source for assessing plant genetic resources by improving our understanding on the distribution and extent of genetic variability present within and among the species. In this study, genetic diversity among and between 16 parents and 12 hybrids of Populus deltoides were analyzed by SSRs (simple sequence repeats). The 18 selected SSR primers yielded 51 polymorphic scorable bands with PIC value of 0.77. The effective multiplex ratio and marker index were 1.82 and 0.80, respectively. Genetic similarity among the collections varied from 0.39 to 0.88 when pooled data of parents and hybrids were analyzed through UPGMA. The analysis of combined data set with SSR, clustered hybrids on dendrogram based on their relationship with parents. Thus, high polymorphism obtained indicates that SSR markers have potential for identification and characterization of genetic variation within and among Populus species.
Genetic diversity, germplasm, hybrids, molecular markers, Populus deltoids, simple sequence repeat
