Applied Biological Research

  • Year: 2020
  • Volume: 22
  • Issue: 1

Parental polymorphism in iron- and zinc-rich rice varieties (Swarna and type 3) Using ssr markers

  • Author:
  • D. Shivani1,2, K. Suman1, P. Madhubabu1, Ramya Rathod2, C. Cheralu2, V. Gouri Shankar2, C.N. Neeraja1,
  • Total Page Count: 7
  • Published Online: Apr 23, 2020
  • Page Number: 69 to 75

1Indian Institute of Rice Research, Rajendranagar, Hyderabad-500 030, Telangana (India)

2Department of Genetics & Plant Breeding, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad-500 030, Telangana (India)

Abstract

A microsatellite is a specific sequence of DNA bases which contains repeats. In present study, rice microsatellite (RM) markers were used to study the parental polymorphism between two parents. The experiment was conducted at Indian Institute of Rice Research, Rajendranagar Hyderabad (India). ‘Swarna’ and ‘Type 3’ rice varieties dense in grain iron and zinc were used to study the parental polymorphism. Out of 171 rice markers used for parental polymorphism, 52 markers showed polymorphism and 119 were monomorphic with expected base pair ranging from 84 to 383 bp. Therefore, these polymorphic rice microsatellite markers can be used in fine mapping of iron- and zinc-rich micronutrient genes to study the mapping populations of crosses obtained from these parents.

Keywords

Biofortification, Iron, Microsatellite markers, Parental polymorphism, Rice, Zinc