Statistical Optimization of Process Parameters for Polygalacturonase Production By Bacillus subtilis KP10B

In present study the statistical optimization of process parameters for costeffective production of polygalacturonase (PGase) by Bacillus subtilis was examined by using Plackett-Burman design (PBD) and central composite design (CCD). Of the 9 factors, temperature, CaCO₃ and KH₂PO₄ were found significant by PBD and were further optimized by CCD. After optimization, maximal PGase activity (272.87 Um L^-1) was achieved at 40°C, 3.5 mM concentration of KH₂PO₄ and 6.5 mM concentration of CaCO₃. The results showed 2.95-fold increase in PGase activity of B. subtilis under optimized conditions. Thus, it may be concluded that optimized medium is cost-effective and suitable for maximum production and may be used for large-scale PGase production by B. subtilis KP10B. The crude enzyme extracts of B. subtilis can be used in industry for fruit juice clarification, waste water treatment and coffee fermentation.