Applied Biological Research
  • Year: 2022
  • Volume: 24
  • Issue: 3

In vitro Evaluation of Antidiabetic, Antioxidant and Probiotic Attributes of Lactobacillus casei strain NCDC 357

1Department of Microbiology, CCS Haryana Agricultural University, Hisar - 125 004, Haryana (India)

Department of Microbiology, Kurukshetra University, Kurukshetra - 136 119, Haryana (India)

*e-mail: baljeet@kuk.ac.in; baljeetsaharan@hau.ac.in

Online Published on 30 September, 2022.

Abstract

The present study was aimed to evaluate the antidiabetic and antioxidant activities of a lactic acid bacterial strain ‘Lactobacillus casei NCDC 357’, and to investigate its probiotic characteristics. The antidiabetic activity was examined by evaluating the α-glucosidase and a-amylase inhibitory activities. L. casei strain NCDC 357 exhibited antidiabetic activities by α-glucosidase (67.7% and IC50 38.08 μL) and a-amylase (53.6% and IC50 49.96 μL). The strain showed antioxidant activities by DPPH (53.4% and IC50 77.60 μL) and ABTS (48.7% and IC50 51.48 μL). Further, the strain was resistant to simulated gastrointestinal juice and showed potential for health promotion based on adhesion, antibiotic susceptibility, and anti­microbial activities. L. casei NCDC 357 appeared to be a potential probiotic having beneficial health effects.

Keywords

Antidiabetic activity, Antioxidant activity, Lactobacillus casei, Probiotic, Strain evaluation