Analysis of the biotechnological cotton plant population for genomes free of NPT II gene

The article deals with the modern technologies and methods for breeding transgenic plants free of selective marker enes (SMG). The classification of the widely applicable methods in the world is given in the paper. The bio-prospecting methodology is presented to identify kanamycingenefree genotypes from the Porlock-1 cotton population. The plasmid vectors designed for transformation including genes for identifying transform ants. As a rule, they are antibiotic resistance genes (for example, non-metallicphospho transferasegenes(npt) and hygromycin phospho transferase genes (hpt)), which products provide transformed plant tissue growth in-selective environment. They have been named as selectable marker genes (SMG)due to their basic function performed in the process of the genetic engineering works. However, after transformed cell screening, the genes lose their value, but remain in human genome transform-ants. Since such sequences started to be called the “genetic burden” and even “genetic debris”, it has become urgent to remove these genes from a plant genome. This kind of research of a cotton plant is carried out for the first time in our country, just as the RNA interference technology for cotton plant, which has changed the qualitative characteristics of the fiber, is used for the first time.