Total Phenolic, Total Flavonoids, Antioxidant and Antifungal Activity of Inula viscosaExtracts from Turkey

This study was conducted to investigate antioxidant, total phenolic, and total flavonoid of ethyl acetate, methanol and hexane extracts of Inula viscosa and the antifungal activity of methanol extract. In the antioxidant study, Free Radical Scavenging Activity (DPPH), ABTS (2,2-azinobis- (3-ethylbenzothiazoline- 6-sulphonic acid), Ferric Reducing Antioxidant Power Activity (FRAP), Cupric Reducing Antioxidant Capacity Activity (CUPRAC), Total phenolic and Flavonoid analyses of each extract were performed. The antioxidant results were calculated at rates ranging between lC₅₀ 10.53-157.07 (gg/mL) for DPPH, lC₅₀ 4.7020.38 (gg/mL) for ABTS, 0.59-4.28 (gmol TE/mg extracts) for FRAP, and 0.23-7.40 (gmol TE/mg extracts) for CUPRAC. The total phenolic and total flavonoid contents of the extracts were determined to be between 11.38-136.18 (mg GAE/g extract) and 14.38-28.83 (mg QE/g Extract). The antifungal activity of the methanol extract of Inula viscosa against Rhizoctonia solani (R.S), Sclerotinia sclerotiorum (S.S), Fusarium oxysporum f sp. melonis (FOM), and Fusarium. oxysporum f sp. cucumerinum (FOC). Agar diffusion method was used in the activity studies. The activity levels of the 1000, 2000 and 4000 ppm doses of the extract against pathogens were determined. Also, it inhibited the FOC, FOM, SS and RS mycelial growths at the rates of 68.23%, 72.04%, 83.51% and 100% at a high dose, 400 ppm. It is considered that the results obtained in this study are important as they guide the development of new natural antifungal substances.