1Sardarbhagwansingh post graduate institute of biomedical science and research, Balawala, Dehradun, Uttarakhand
2Department of Botany, D.D.U. Gorakhpur University, Gorakhpur, U.P, India
*Corresponding author: renumaurya@gmail.com
Online published on 31 August, 2017.
Symptoms of yellowing, little leaf and witches’ broom on Cannabis sativa L. ssp. sativa plants were observed in Dehradun, Uttarakhand, India during June-July 2016. Symptomatic plants yielded phytoplasma DNA bands of ∼1.2 kb from nested PCR assays using universal phytoplasma primers P1/P7 and R16F2n/R16R2. BLASTn search analysis of C. sativa sp. phytoplasma isolates shared 99% of sequence identity with phytoplasma isolates belongs to 16SrXIV group (Candidatus Phytoplasma cynodontis). Phylogenetic analysis of the 16SrDNA sequences of C. sativa phytoplasma isolates revealed their closest relationship with members of the 16SrXIV group. Real and virtual RFLP analysis of the 16SrDNA sequences of Can LL-WB phytoplasma isolates confirmed that the belonged to the 16SrXIV group.
Nested PCR assay, Phylogenetic analysis, RFLP ‘Ca. P. cynodontis’
