Agrica
  • Year: 2017
  • Volume: 6
  • Issue: 2

Bioinoculants from-Azospirillum brasilense (Dobereiner, 1978), Bacillus megaterium (Ehrenberg, 1835) var. phosphaticum and Pseudomonas fluorescens (Migula, 1895) and its storability

1Assistant Professor (Agricultural Microbiology), Plant Pathology unit, Tamil Nadu Rice Research Institute, Aduthurai-612101.

2 Professor, Dept. of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore

3Assistant Professor (Plant Pathology), Plant Pathology unit, Tamil Nadu Rice Research Institute, Aduthurai-612101

*Corresponding author: jbharathi86@gmail.com,

**dbalu2000@gmail.com,

***ahila.devi1@gmail.com,

Online published on 5 April, 2018.

Abstract

Use of carrier based inoculum for crop growth and yield is wide practice among the conventional agriculture. Precision farming is a site specific management approach. Carrier based inoculum is not suitable for precision farming system due to clogging effect. Hence, liquid bioinoculant's was prepared using N fixer (Azospirillum brasilense), P solubilizer (Bacillus megaterium var. phosphaticum) and PGPR (Pseudomonas fluorescens). The results of the experiment convey that the inoculants supplemented with 10mM of phosphate buffer (pH 7) + 10% glycerol supported maximum survival of Azospirillum (0.17 x 1019 MPN/mL), Bacillus (25 x 1019 cfu/mL) and Pseudomonas (21 x 1019 cfu/mL) at room temperature.

Keywords

N fixer, P solubilizer, PGPR, cell protectants, storage