1Department of Botany, C.C.R (PG) College, Muzaffarnagar (U.P.), India
2Department of Ag. Botany, C.C.R (PG) College, Muzaffarnagar (U.P.), India
3Department of Botany, Meerut College, Meerut (U.P.), India
4Department of Botany, D.N. College, Meerut (U.P.), India
5Department of Botany, Meerut College, Meerut (U.P.), India
*Corresponding Author E-mail– shrikant2704@rediffmail.com
Online Published on 20 June, 2023.
Recent advances in Molecular biology, Genomics, Proteomics, Bioinformatics and Statistical tools (quantitative genetics), made it possible to dissect the genomic regions associated(QTLs/genes) with stress tolerance in food crops. The forward genetics tools like fine mapping,map based cloning, insertional mutagenesis and random mutations are also being used tounravel the genetic basis of phenotypic variations which lead to development of abiotic stresstolerance/resistance characters in crops. Today genome sequencing is very cost effective. Costeffective sequencing tools enable us to decode the sequences of coding regions of genome fordifferent abiotic stress responses using microarray technology. Reverse Genetic approach withthe help of advanced molecular biology tools such as RNAi, CRISPR/CAS genome editing,TIILLING, Antisense RNA etc. also simplify the decoding of the function of genes by analysingphenotypic variations due to different abiotic stresses. Further, bioinformatics tools help us toanalyse the massive amount of data or information generated in last decade from ‘omic’ tools.This data provides a basis for identification of more functional genes, which could contributedirectly to cellular drought stress tolerance/ resistance.
Abiotic stress, Genomics, Climate change, Stress tolerance varieties
