1Department of Botany, University of Kashmir Srinagar, India.
2Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar – Punjab, India
Online published on 18 May, 2012.
Calli were induced from corolla explants of Petunia hybrida var. Nana Compacta on MS medium supplemented with both auxins and cytokinins. Among the auxins, NAA (10.7µM/l) was most effective in inducing a white and creamy callus. Maximum callus multiplication rates (34.7%) were observed on MS medium supplemented with 10.7µM/l NAA.16–18 shoots were induced from the corolla derived callus on MS medium supplemented with 8.9µM/l BA. The regenerated shoots formed roots when cultured on MS medium supplemented with a combination of 5.4µM/l NAA + 4.9µM/l IBA. Young plantlets were successfully transferred to community pots for field evaluations.
Petunia hybrida, auxins, callus induction, corolla explants, root formation
