1College of Biotechnology, S.V.P.U.A. & T., Meerut-250110
2College of Agriculture, S.V.P.U.A. & T., Meerut-250110
3Department of Biotechnology and Microbiology, Gandhi-Faiz-e-Aam College, Shahjahanpur-242001, Uttar Pradesh
Online published on 18 May, 2012.
The present study was carried out to study the effect of different phytohormones on rate of multiplication of Cucurbita maxima during in vitro micropropagation. Shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with different BAP, Kinetin and NAA at various levels of concentration and their combination for shoot proliferation. The proliferation rate, shoot quality and other parameters studied indicate that the optimal treatment was 2 mg/l BAP. Shoots were rooted most effectively in half strength MS medium supplemented with 5mg/l NAA. Rooted plants were hardened and successfully established in soil with 90% survival rate.
Cucurbita maxima, shoot tip explant, shoot proliferation
