The State Key Laboratory of Soil Erosion and Dryland Farm of Loess Plateau Institute of Soil and Water Conservation, Chinese Academy of Sciences, Northwest University of Agriculture and Forestry, Yangling-712100, Shaanxi, China.
2College of Life Science, Northwest University of Agriculture and Forestry, Yangling, Shaanxi 712100, China.
3Graduate School of Chinese Academy of Sciences, Beijing 100039, China.
*Correspondence author. Phone: +86–29-87001011, Fax: + 86–29-87012210; E-mail: mayongqing@ms.iswc.ac.cn
The effect of cultured A. adsurgens roots on the cultured shoot proliferation and root inducement of Stelleria chamaejasme L was investigated by conducting a bioassay using the filtrate extract from A. adsurgens root culture partitioned with ethyl acetate. It showed that proliferation, elongation and dry weight accumulation of S. chamaejasme cultured shoots were higher in the treatment containing 3.2 mg/L of filtrate extract compared to the control (0 mg/L). The proliferation, elongation, fresh weight and dry weight accumulation of cultured shoots from S. chamaejasme were inhibited at 6.4, 12.7, and 25.4 mg/L of the filtrate extract. The rooting rate of S. chamaejasme cultured shoots was significantly lower at 30 d in the treatments containing 12.7 and 25.4 mg/L of culture filtrate extract compared to the control. This study indicated that the inhibitory and stimulatory effects of ethyl acetate extracts of A. adsurgens roots were due to chemicals secreted by its cultured roots. This study proves that A. adsurgens has allelopathic potential on S. chamaejasme.
Allelopathic potential, cultured Stelleria chamaejasme L, culture filtrate extract, prairie milk vetch (Astragalus adsurgens Pall), root inducement, shoot proliferation
