Chemical inducement of 2,4-dihyroxy-7-methoxy-1, 4-benzoxazin-3-one (DIMBOA) in wheat seedlings

HPLC quantitative analysis of 2,4-dihyroxy-7-methoxy-1, 4-benzoxazin-3-one (DIMBOA) in seedling of 3 wheat varieties (Zhongfu 9507, Jing 411 and Zhongbeizhong 39) shoots showed that DIMBOA concentration could be induced by several chemicals [Methyl jasmonate (JA), methyl salicylate (SA), triadimefon, potassium dihydrogen phosphate (KH₂PO₄) and cupric chloride (CuCl₂)] in different concentrations. The inducement effects on DIMBOA content in hydroponic solution were not obvious, but the inducement effects on DIMBOA production of shoot in wheat seedlings followed the order: JA > SA > triadimefon > KH₂PO₄ > CuCl₂. Common inducers such as JA and SA showed significant inducement effects, whereas, the effects of systemic fungicide triadimefon and the fertilizer KH₂PO₄ were weak. A heavy metal ion CuCl₂, showed inhibitory effects on Zhongfu 9507. The optimal concentration of JA is 561.0 μg/ml (2.5 μmol/ml), which was much higher than any other treatments.