1College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
2Kurdistan University, College of Agriculture, Agronomy Dept., Sanandaj, Iran.
3Pest and Disease Research Institute, Tehran, Iran.
*Corresponding author: E-mail: sedighk@gmail.com
It is the first report of walnut mycorrhizal fungi. We studied diversity of Glomus species fungi in walnut rhizosphere. Sixty eight soil samples were collected from walnut rhizosphere, mycorrhizal fungi spores were extracted from the soil and the species were identified by spores morphological properties. The high-resolution fingerprinting method of amplified fragment length polymorphism (AFLP) was used to assess the genetic diversity analysis of mycorrhizal isolates. According to our molecular and morphological analysis, AFLP proved suitable marker for molecular genetic classification of mycorrhizal fungi. Of the 274 obtained isolates, 264 were related to Glomus genus (15 spp), 4 to Paraglomus (1 sp) and 6 belong to Gigaspora genus (1 sp).
AFLP marker, genetic diversity, Glomus species, rhizosphere, walnut
