College of Life Sciences, Nankai University, Tianjin 300071, China. E. Mail: ruanweibin2004@hotmail.com.
*Correspondence author
1College of Resources and Environment, China Agricultural University, Beijing 100094, China.
The application of m-hydroxy-phenylacetic acid (MHPA), significantly inhibited the radicle growth and root tips became thicker and brownish in colour than untreated control. Transmission electron microscopy analysis showed that MHPA adversely affected the soybean root tip, leading to sparse cytoplasm and fewer organelles in root meristematic cell, cell vacuolation and poorly developed irregular amyloplasts in root columella cell. A total of 33 differentially displayed cDNA fragments were found between the MHPA treated and control samples and 6 differential displayed cDNA fragments were observed after the subsequent DDRT-PCR reactions on extracted RNA. Results from RT-PCR confirmed two differential expressions, (i). one down-regulated gene (S385) and (ii). one up-regulated gene (S452), these expressed approximate half-fold and 5-folds over the control plants, respectively.
Autotoxicity, differential gene expression, Glycine max L. Merr., m-hydroxy-phenylacetic acid (MHPA), phenolic acids, radicle, real-time RT-PCR, soybean, ultrastructure
