Effects of water-soluble compounds of Polygonatum cirrhifolium rhizomes on plant pathogenic fungi
Abstract
Three water-soluble compounds were isolated and characterized from the rhizomes of Polygonatum cirrhifolium. Their structures were determined as (25R)spirost-5-ene-3β-ol-3-O-α-L-rhamnopyranosyl(1→2)-[α-L-rhamnopyranosyl (1→4)]β-D-glucopyranoside (A), (25R)-spirost-5-ene-3β-ol-3-O-[α-L-rhamnopyranosyl (1→4)]-β-D-glucopyranoside (B) and n-butyl-β-D-fructopyranoside (C). These compounds were isolated first time from P. cirrhifolium and their antifungal activity was tested against growth of 11 plant pathogenic fungi. The compound A (3-sugars linked at C-3) drastically inhibited the growth of Exserohilum turcicum and Botryosphaeria ribis than compound B (2-sugars linked at C-3), with EC50 of 46.8 and 57.5 μg/ml, respectively. Diosgenin (the aglycone of compound A and B) was also more inhibitory to growth of Fusarium oxysporum with EC50 of 288.4 μg/ml.
Keywords
Alternaria, antifungal activity, Botryosphaeria ribis, brassicae, Colletotrichum lagenarium, Colletotrichum gloeosporioides, Cytospora chrysosperma, Exserohilum turcicum, Fusarium graminearum, Fusarium oxysporum, isolation and identification, Polygonatum cirrhifolium, Trichothecium roseum, Verticillium dahliae, water-soluble compounds